7EJP

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltohexaose

Summary for 7EJP

• Entry DOI: 10.2210/pdb7ejp/pdb
• Descriptor: 4-alpha-glucanotransferase, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: glycogen debranching enzyme, sugar binding protein
• Biological source: Candida glabrata CBS 138 (Yeast, Torulopsis glabrata)
• Total number of polymer chains: 2
• Total formula weight: 354209.72

Authors

Shen, M.,Xiang, S. (deposition date: 2021-04-02, release date: 2021-11-10, Last modification date: 2023-11-29)

Primary citation

Shen, M.,Gong, X.,Xiang, S.
Crystal structures of glycogen-debranching enzyme mutants in complex with oligosaccharides.
Acta Crystallogr.,Sect.F, 77:420-426, 2021

PubMed Abstract: Debranching is a critical step in the mobilization of the important energy store glycogen. In eukaryotes, including fungi and animals, the highly conserved glycogen-debranching enzyme (GDE) debranches glycogen by a glucanotransferase (GT) reaction followed by a glucosidase (GC) reaction. Previous work indicated that these reactions are catalyzed by two active sites located more than 50 Å apart and provided insights into their catalytic mechanisms and substrate recognition. Here, five crystal structures of GDE in complex with oligosaccharides with 4-9 glucose residues are presented. The data suggest that the glycogen main chain plays a critical role in binding to the GT and GC active sites of GDE and that a minimum of five main-chain residues are required for optimal binding.

PubMed: 34726181
DOI: 10.1107/S2053230X21010918

Experimental method

X-RAY DIFFRACTION (3.1 Å)