7EJP

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltohexaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	4-alpha-glucanotransferase	polymer	1536	175429.4	2	UniProt (Q6FSK0) Pfam (PF14699) Pfam (PF14701) Pfam (PF14702) Pfam (PF06202) In PDB	Candida glabrata CBS 138 (Yeast Torulopsis glabrata)	Amylo-alpha-1,6-glucosidase,Dextrin 6-alpha-D-glucosidase,Glycogen debrancher,Glycogen debranching enzyme,Oligo-1,4-1,4-glucantransferase
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB GlyTouCan (G07411ON)
3	E, F	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB GlyTouCan (G96370VA)
4	G, H	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		828.7	2	In PDB GlyTouCan (G50146AM)

Sequence modifications

A, B: 1 - 1528 (UniProt: Q6FSK0)

PDB	External Database	Details
Ala 470	Trp 470	engineered mutation
Leu 1529	-	expression tag
Glu 1530	-	expression tag
His 1531	-	expression tag
His 1532	-	expression tag
His 1533	-	expression tag
His 1534	-	expression tag
His 1535	-	expression tag
His 1536	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	350858.8
Branched	Number of molecules	6
	Total formula weight	3350.9
All*	Total formula weight	354209.7

*Water molecules are not included.