7EJP
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltohexaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91769 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.537, 199.828, 133.878 |
Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
Resolution | 38.440 - 3.100 |
R-factor | 0.2369 |
Rwork | 0.236 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d0f |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.440 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.067 | |
Number of reflections | 73954 | 3680 |
<I/σ(I)> | 15.74 | |
Completeness [%] | 99.8 | |
Redundancy | 3.8 | |
CC(1/2) | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride |