7BVH
Crystal structure of arabinosyltransferase EmbC2-AcpM2 complex from Mycobacterium smegmatis complexed with di-arabinose
Summary for 7BVH
| Entry DOI | 10.2210/pdb7bvh/pdb |
| Related PRD ID | PRD_900018 |
| Descriptor | Integral membrane indolylacetylinositol arabinosyltransferase EmbC, Meromycolate extension acyl carrier protein, alpha-D-arabinofuranose-(1-5)-alpha-D-arabinofuranose, ... (7 entities in total) |
| Functional Keywords | cell wall synthesis, arabinosyltransferase, drug target, transferase |
| Biological source | Mycolicibacterium smegmatis MC2 155 More |
| Total number of polymer chains | 4 |
| Total formula weight | 264975.47 |
| Authors | |
| Primary citation | Zhang, L.,Zhao, Y.,Gao, Y.,Wu, L.,Gao, R.,Zhang, Q.,Wang, Y.,Wu, C.,Wu, F.,Gurcha, S.S.,Veerapen, N.,Batt, S.M.,Zhao, W.,Qin, L.,Yang, X.,Wang, M.,Zhu, Y.,Zhang, B.,Bi, L.,Zhang, X.,Yang, H.,Guddat, L.W.,Xu, W.,Wang, Q.,Li, J.,Besra, G.S.,Rao, Z. Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol. Science, 368:1211-1219, 2020 Cited by PubMed Abstract: The arabinosyltransferases EmbA, EmbB, and EmbC are involved in cell wall synthesis and are recognized as targets for the anti-tuberculosis drug ethambutol. In this study, we determined cryo-electron microscopy and x-ray crystal structures of mycobacterial EmbA-EmbB and EmbC-EmbC complexes in the presence of their glycosyl donor and acceptor substrates and with ethambutol. These structures show how the donor and acceptor substrates bind in the active site and how ethambutol inhibits arabinosyltransferases by binding to the same site as both substrates in EmbB and EmbC. Most drug-resistant mutations are located near the ethambutol binding site. Collectively, our work provides a structural basis for understanding the biochemical function and inhibition of arabinosyltransferases and the development of new anti-tuberculosis agents. PubMed: 32327601DOI: 10.1126/science.aba9102 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.3 Å) |
Structure validation
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