6ZUI
Crystal structure of the Cys-Ser mutant of the cpYFP-based biosensor for hypochlorous acid
Summary for 6ZUI
| Entry DOI | 10.2210/pdb6zui/pdb |
| Descriptor | HTH-type transcriptional repressor NemR,Green fluorescent protein,Green fluorescent protein,HTH-type transcriptional repressor NemR (2 entities in total) |
| Functional Keywords | biosensor, hypohalous acid, cpyfp, yellow fluorescent protein, fluorescent protein |
| Biological source | Escherichia coli (strain K12) More |
| Total number of polymer chains | 1 |
| Total formula weight | 49632.64 |
| Authors | Tossounian, M.A.,Van Molle, I.,Messens, J. (deposition date: 2020-07-23, release date: 2021-06-30, Last modification date: 2024-10-16) |
| Primary citation | Kostyuk, A.I.,Tossounian, M.A.,Panova, A.S.,Thauvin, M.,Raevskii, R.I.,Ezerina, D.,Wahni, K.,Van Molle, I.,Sergeeva, A.D.,Vertommen, D.,Gorokhovatsky, A.Y.,Baranov, M.S.,Vriz, S.,Messens, J.,Bilan, D.S.,Belousov, V.V. Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives. Nat Commun, 13:171-171, 2022 Cited by PubMed Abstract: The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 10 Ms range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model. PubMed: 35013284DOI: 10.1038/s41467-021-27796-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.20008218184 Å) |
Structure validation
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