6ZUI
Crystal structure of the Cys-Ser mutant of the cpYFP-based biosensor for hypochlorous acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | HTH-type transcriptional repressor NemR,Green fluorescent protein,Green fluorescent protein,HTH-type transcriptional repressor NemR | polymer | 448 | 49632.6 | 1 | UniProt (P67430) UniProt (P42212) Pfam (PF00440) Pfam (PF16925) Pfam (PF01353) | Escherichia coli (strain K12) | Bleach-sensing transcription factor,Redox-regulated transcription factor NemR,Bleach-sensing transcription factor,Redox-regulated transcription factor NemR |
| 2 | B (A) | water | water | 18.0 | 185 | Chemie (HOH) |
Sequence modifications
A: 1 - 102 (UniProt: P67430)
A: 106 - 198 (UniProt: P42212)
A: 209 - 351 (UniProt: P42212)
A: 354 - 448 (UniProt: P67430)
| PDB | External Database | Details |
|---|---|---|
| Ser 21 | Cys 21 | engineered mutation |
| Ser 98 | Cys 98 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 109 | His 148 | engineered mutation |
| Ala 124 | Val 163 | engineered mutation |
| Val 132 | Ile 171 | engineered mutation |
| Phe 164 | Thr 203 | engineered mutation |
| Val 167 | Ala 206 | engineered mutation |
| Leu 192 | His 231 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 199 | - | linker |
| Val 200 | - | linker |
| Asp 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Ser 204 | - | linker |
| Gly 205 | - | linker |
| Gly 206 | - | linker |
| Thr 207 | - | linker |
| Gly 208 | - | linker |
| Leu 253 | Phe 46 | engineered mutation |
| Leu 271 | Phe 64 | engineered mutation |
| Cr2 273 | Ser 65 | chromophore |
| - | Tyr 66 | chromophore |
| - | Gly 67 | chromophore |
| Leu 275 | Val 68 | engineered mutation |
| Lys 276 | Gln 69 | engineered mutation |
| Ala 279 | Ser 72 | engineered mutation |
| Gly 336 | Asp 129 | engineered mutation |
| Gly 352 | - | linker |
| Thr 353 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ser 103 | - | linker |
| Ala 104 | - | linker |
| Gly 105 | - | linker |
| Ser 355 | Cys 106 | engineered mutation |
| Ser 365 | Cys 116 | engineered mutation |
| Ser 398 | Cys 149 | engineered mutation |
| Ser 402 | Cys 153 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 49632.6 | |
| All* | Total formula weight | 49632.6 |
