6ZOO

Photosystem I reduced Plastocyanin Complex

6ZOO の概要

• エントリーDOI: 10.2210/pdb6zoo/pdb
• 関連するPDBエントリー: 6YEZ
• EMDBエントリー: 11326
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit X psaK, ... (33 entities in total)
• 機能のキーワード: photosystem i, membrane protein, photosynthesis
• 由来する生物種: Pisum sativum (Garden pea); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 563158.92

構造登録者

Nelson, N.,Caspy, I.,Shkolnisky, Y. (登録日: 2020-07-07, 公開日: 2021-06-16, 最終更新日: 2025-04-09)

主引用文献

Caspy, I.,Fadeeva, M.,Kuhlgert, S.,Borovikova-Sheinker, A.,Klaiman, D.,Masrati, G.,Drepper, F.,Ben-Tal, N.,Hippler, M.,Nelson, N.
Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions.
Biochem.J., 478:2371-2384, 2021

PubMed Abstract: Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover.

PubMed: 34085703
DOI: 10.1042/BCJ20210267

実験手法

ELECTRON MICROSCOPY (2.74 Å)