6ZKZ
Crystal structure of InhA:01 TCR in complex with HLA-E (F116C) bound to InhA (53-61 H4C)
Summary for 6ZKZ
| Entry DOI | 10.2210/pdb6zkz/pdb |
| Descriptor | HLA class I histocompatibility antigen, alpha chain E, Beta-2-microglobulin, Enoyl-[acyl-carrier-protein] reductase [NADH], ... (7 entities in total) |
| Functional Keywords | t cell receptor, tcr, hla-e, inha, immune system |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 5 |
| Total formula weight | 93908.15 |
| Authors | Srikannathasan, V.,Karuppiah, V.,Robinson, R.A. (deposition date: 2020-06-30, release date: 2022-01-26, Last modification date: 2024-02-07) |
| Primary citation | Barber, C.,De Souza, V.A.,Paterson, R.L.,Martin-Urdiroz, M.,Mulakkal, N.C.,Srikannathasan, V.,Connolly, M.,Phillips, G.,Foong-Leong, T.,Pengelly, R.,Karuppiah, V.,Grant, T.,Dembek, M.,Verma, A.,Gibbs-Howe, D.,Blicher, T.H.,Knox, A.,Robinson, R.A.,Cole, D.K.,Leonard, S. Structure-guided stabilization of pathogen-derived peptide-HLA-E complexes using non-natural amino acids conserves native TCR recognition. Eur.J.Immunol., 52:618-632, 2022 Cited by PubMed Abstract: The nonpolymorphic class Ib molecule, HLA-E, primarily presents peptides from HLA class Ia leader peptides, providing an inhibitory signal to NK cells via CD94/NKG2 interactions. Although peptides of pathogenic origin can also be presented by HLA-E to T cells, the molecular basis underpinning their role in antigen surveillance is largely unknown. Here, we solved a co-complex crystal structure of a TCR with an HLA-E presented peptide (pHLA-E) from bacterial (Mycobacterium tuberculosis) origin, and the first TCR-pHLA-E complex with a noncanonically presented peptide from viral (HIV) origin. The structures provided a molecular foundation to develop a novel method to introduce cysteine traps using non-natural amino acid chemistry that stabilized pHLA-E complexes while maintaining native interface contacts between the TCRs and different pHLA-E complexes. These pHLA-E monomers could be used to isolate pHLA-E-specific T cells, with obvious utility for studying pHLA-E restricted T cells, and for the identification of putative therapeutic TCRs. PubMed: 35108401DOI: 10.1002/eji.202149745 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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