6ZK8

Native crystal structure of anaerobic F420H2-Oxidase from Methanothermococcus thermolithotrophicus at 1.8A resolution

Summary for 6ZK8

Entry DOI	10.2210/pdb6zk8/pdb
Descriptor	Coenzyme F420H2 oxidase (FprA), DI(HYDROXYETHYL)ETHER, PENTAETHYLENE GLYCOL, ... (7 entities in total)
Functional Keywords	oxidase, four electron reduction, oxygen, anaerobic, oxidoreductase
Biological source	Methanothermococcus thermolithotrophicus
Total number of polymer chains	2
Total formula weight	95658.34
Authors	Engilberge, S.,Wagner, T.,Carpentier, P.,Girard, E.,Shima, S. (deposition date: 2020-06-30, release date: 2020-11-25, Last modification date: 2024-01-31)
Primary citation	Engilberge, S.,Wagner, T.,Carpentier, P.,Girard, E.,Shima, S. Krypton-derivatization highlights O 2 -channeling in a four-electron reducing oxidase. Chem.Commun.(Camb.), 56:10863-10866, 2020 Cited by PubMed Abstract: F420H2-oxidase (FprA) catalyses the four-electron reduction of O2 to 2H2O using the reduced form of F420 as electron donor. The hydrophobic O2-channel detected by Kr-derivatization and the concerted movement of a gating loop could contribute to prevent unwanted side-reaction between the catalytic intermediates and solvents, therefore preventing reactive oxygen species formation. PubMed: 32940290 DOI: 10.1039/d0cc04557h PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.83 Å)

Structure validation