6ZK8
Native crystal structure of anaerobic F420H2-Oxidase from Methanothermococcus thermolithotrophicus at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.83 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.871, 156.609, 76.065 |
| Unit cell angles | 90.00, 137.62, 90.00 |
Refinement procedure
| Resolution | 42.890 - 1.830 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6frm |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.040 | 1.990 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.094 | 0.815 |
| Rpim | 0.051 | 0.456 |
| Number of reflections | 47259 | 2363 |
| <I/σ(I)> | 7.7 | 1.3 |
| Completeness [%] | 88.8 | |
| Redundancy | 3.1 | 2.7 |
| CC(1/2) | 0.993 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M TRIS pH 8.5, 40% PEG 400, 0.2M Lithium Sulfate |
