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6YW1

HIF prolyl hydroxylase 2 (PHD2/ EGLN1) in complex with 2OG and RaPID-derived silent allosteric cyclic peptide 3C (14-mer)

Summary for 6YW1
Entry DOI10.2210/pdb6yw1/pdb
Related5L9B 5L9R 5L9V
DescriptorEgl nine homolog 1, PHD2-SPECIFIC RaPID CYCLIC PEPTIDE 3C (14-MER), MANGANESE (II) ION, ... (6 entities in total)
Functional Keywordsoxidoreductase, non-heme dioxygenase, iron, 2-oxoglutarate, hypoxia-inducible factor, hif, hif prolyl hydroxylase domain 2, phd2, egln1, oxygenase, hypoxia, dna-binding, metal-binding, transcription, helix-loop-helix-beta, dsbh, facial triad, cytoplasm, transcription/epigenetic regulation, signaling, development, cell structure, beta-hydroxylation, transcription activator/inhibitor, ubl conjugation, polymorphism, vitamin c, zinc-finger, familial erythrocytosis, breast cancer, transcription complex
Biological sourceHomo sapiens (Human)
More
Total number of polymer chains2
Total formula weight28099.68
Authors
Chowdhury, R.,Schofield, C.J. (deposition date: 2020-04-29, release date: 2020-12-30, Last modification date: 2024-01-24)
Primary citationChowdhury, R.,Abboud, M.I.,McAllister, T.E.,Banerji, B.,Bhushan, B.,Sorensen, J.L.,Kawamura, A.,Schofield, C.J.
Use of cyclic peptides to induce crystallization: case study with prolyl hydroxylase domain 2.
Sci Rep, 10:21964-21964, 2020
Cited by
PubMed Abstract: Crystallization is the bottleneck in macromolecular crystallography; even when a protein crystallises, crystal packing often influences ligand-binding and protein-protein interaction interfaces, which are the key points of interest for functional and drug discovery studies. The human hypoxia-inducible factor prolyl hydroxylase 2 (PHD2) readily crystallises as a homotrimer, but with a sterically blocked active site. We explored strategies aimed at altering PHD2 crystal packing by protein modification and molecules that bind at its active site and elsewhere. Following the observation that, despite weak inhibition/binding in solution, succinamic acid derivatives readily enable PHD2 crystallization, we explored methods to induce crystallization without active site binding. Cyclic peptides obtained via mRNA display bind PHD2 tightly away from the active site. They efficiently enable PHD2 crystallization in different forms, both with/without substrates, apparently by promoting oligomerization involving binding to the C-terminal region. Although our work involves a specific case study, together with those of others, the results suggest that mRNA display-derived cyclic peptides may be useful in challenging protein crystallization cases.
PubMed: 33319810
DOI: 10.1038/s41598-020-76307-8
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.46 Å)
Structure validation

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