6YBT

Structure of a human 48S translational initiation complex - eIF3bgi

Summary for 6YBT

• Entry DOI: 10.2210/pdb6ybt/pdb
• EMDB information: 10773
• Descriptor: Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit I, Eukaryotic translation initiation factor 3 subunit B (3 entities in total)
• Functional Keywords: eif3, ribosome, translation, initiation complex
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 3
• Total formula weight: 292316.72

Authors

Brito Querido, J.,Sokabe, M.,Kraatz, S.,Gordiyenko, Y.,Skehel, M.,Fraser, C.,Ramakrishnan, V. (deposition date: 2020-03-17, release date: 2020-09-16, Last modification date: 2024-05-22)

Primary citation

Brito Querido, J.,Sokabe, M.,Kraatz, S.,Gordiyenko, Y.,Skehel, J.M.,Fraser, C.S.,Ramakrishnan, V.
Structure of a human 48Stranslational initiation complex.
Science, 369:1220-1227, 2020

PubMed Abstract: A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning.

PubMed: 32883864
DOI: 10.1126/science.aba4904

Experimental method

ELECTRON MICROSCOPY (6 Å)