6YAI

Clathrin with bound beta2 appendage of AP2

6YAI の概要

• エントリーDOI: 10.2210/pdb6yai/pdb
• 関連するPDBエントリー: 6YAE 6YAF 6YAH
• EMDBエントリー: 10747 10748 10749 10750 10751 10752 10753 10754
• 分子名称: Clathrin heavy chain, AP-2 complex subunit beta, Clathrin light chain, ... (4 entities in total)
• 機能のキーワード: clathrin, clathrin adaptor, ap2, clathrin assembly, endocytosis
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 1811581.55

構造登録者

Kovtun, O.,Kane Dickson, V.,Kelly, B.T.,Owen, D.,Briggs, J.A.G. (登録日: 2020-03-12, 公開日: 2020-07-29, 最終更新日: 2024-05-22)

主引用文献

Kovtun, O.,Dickson, V.K.,Kelly, B.T.,Owen, D.J.,Briggs, J.A.G.
Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.
Sci Adv, 6:eaba8381-eaba8381, 2020

PubMed Abstract: Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell's plasma membrane. Clathrin forms a cage-like, polyhedral outer scaffold around a vesicle, to which cargo-selecting clathrin adaptors are attached. Adaptor protein complex (AP2) is the key adaptor in CME. Crystallography has shown AP2 to adopt a range of conformations. Here, we used cryo-electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane-assembled clathrin-coated vesicles (CCVs). AP2 binds cargo and PtdIns(4,5) (phosphatidylinositol 4,5-bisphosphate)-containing membranes via multiple interfaces, undergoing conformational rearrangement from its cytosolic state. The binding mode of AP2 β2 appendage into the clathrin lattice in CCVs and buds implies how the adaptor structurally modulates coat curvature and coat disassembly.

PubMed: 32743075
DOI: 10.1126/sciadv.aba8381

実験手法

ELECTRON MICROSCOPY (9.2 Å)