6Y73

The crystal structure of human MACROD2 in space group P43

6Y73 の概要

• エントリーDOI: 10.2210/pdb6y73/pdb
• 関連するPDBエントリー: 6Y4Y 6Y4Z
• 分子名称: ADP-ribose glycohydrolase MACROD2, GLYCEROL, L(+)-TARTARIC ACID, ... (5 entities in total)
• 機能のキーワード: adp-ribosylhydrolase, macrodomain, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 328621.92

構造登録者

Wazir, S.,Maksimainen, M.M.,Lehtio, L. (登録日: 2020-02-28, 公開日: 2020-09-30, 最終更新日: 2024-01-24)

主引用文献

Wazir, S.,Maksimainen, M.M.,Lehtio, L.
Multiple crystal forms of human MacroD2.
Acta Crystallogr.,Sect.F, 76:477-482, 2020

PubMed Abstract: MacroD2 is one of the three human macrodomain proteins characterized by their protein-linked mono-ADP-ribosyl-hydrolyzing activity. MacroD2 is a single-domain protein that contains a deep ADP-ribose-binding groove. In this study, new crystallization conditions for MacroD2 were found and three crystal structures of human MacroD2 in the apo state were solved in space groups P422, P422 and P4, and refined at 1.75, 1.90 and 1.70 Å resolution, respectively. Structural comparison of the apo crystal structures with the previously reported crystal structure of MacroD2 in complex with ADP-ribose revealed conformational changes in the side chains of Val101, Ile189 and Phe224 induced by the binding of ADP-ribose in the active site. These conformational variations may potentially facilitate design efforts of a MacroD2 inhibitor.

PubMed: 33006575
DOI: 10.1107/S2053230X20011309

実験手法

X-RAY DIFFRACTION (1.7 Å)