6XTV

FULL-LENGTH LTTR LYSG FROM CORYNEBACTERIUM GLUTAMICUM WITH BOUND EFFECTOR ARG

Summary for 6XTV

• Entry DOI: 10.2210/pdb6xtv/pdb
• Related: 6XTU
• Descriptor: Lysine export transcriptional regulatory protein LysG, ARGININE (2 entities in total)
• Functional Keywords: lttr helix-turn-helix transcription regulation, transcription
• Biological source: Corynebacterium glutamicum MB001
• Total number of polymer chains: 2
• Total formula weight: 63021.41

Authors

Hofmann, E.,Syberg, F.,Schlicker, C.,Eggeling, L.,Schendzielorz, G. (deposition date: 2020-01-16, release date: 2020-10-07, Last modification date: 2024-01-24)

Primary citation

Della Corte, D.,van Beek, H.L.,Syberg, F.,Schallmey, M.,Tobola, F.,Cormann, K.U.,Schlicker, C.,Baumann, P.T.,Krumbach, K.,Sokolowsky, S.,Morris, C.J.,Grunberger, A.,Hofmann, E.,Schroder, G.F.,Marienhagen, J.
Engineering and application of a biosensor with focused ligand specificity.
Nat Commun, 11:4851-4851, 2020

PubMed Abstract: Cell factories converting bio-based precursors to chemicals present an attractive avenue to a sustainable economy, yet screening of genetically diverse strain libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor. For this reason, transcriptional biosensors attract attention as they allow the screening of vast libraries when used in combination with fluorescence-activated cell sorting (FACS). However, broad ligand specificity of transcriptional regulators (TRs) often prohibits the development of such ultra-high-throughput screens. Here, we solve the structure of the TR LysG of Corynebacterium glutamicum, which detects all three basic amino acids. Based on this information, we follow a semi-rational engineering approach using a FACS-based screening/counterscreening strategy to generate an L-lysine insensitive LysG-based biosensor. This biosensor can be used to isolate L-histidine-producing strains by FACS, showing that TR engineering towards a more focused ligand spectrum can expand the scope of application of such metabolite sensors.

PubMed: 32978386
DOI: 10.1038/s41467-020-18400-0

Experimental method

X-RAY DIFFRACTION (3.3 Å)