6XTV

FULL-LENGTH LTTR LYSG FROM CORYNEBACTERIUM GLUTAMICUM WITH BOUND EFFECTOR ARG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979657
Spacegroup name	P 41 21 2
Unit cell lengths	123.950, 123.950, 111.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 3.300
Rwork	0.206
R-free	0.25080
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6xtu
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	XDS (VERSION Oct 15, 2015)
Data scaling software	XSCALE (VERSION Oct 15, 2015 BUILT=20151231)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	3.111
High resolution limit [Å]	3.000	3.000
Rmerge	0.130	1.940
Rmeas	0.140	2.084
Rpim	0.051	0.755
Number of reflections	17846	12966
<I/σ(I)>	15.77	1.15
Completeness [%]	99.7
Redundancy	7.2
CC(1/2)	0.999	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M SODIUM CHLORIDE; 0.1 M TRIS PH REMARK 280 8.5, 30 %(V/V) PEG 400