6XH8
CueR-transcription activation complex with RNA transcript
Summary for 6XH8
| Entry DOI | 10.2210/pdb6xh8/pdb |
| Related | 6XH7 |
| EMDB information | 22184 22185 22289 |
| Descriptor | DNA-directed RNA polymerase subunit alpha, ZINC ION, COPPER (II) ION, ... (11 entities in total) |
| Functional Keywords | transcription activation, rna polymerase, merr-family, transcription, transcription-dna-rna complex, transcription/dna/rna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 11 |
| Total formula weight | 529160.31 |
| Authors | |
| Primary citation | Shi, W.,Zhang, B.,Jiang, Y.,Liu, C.,Zhou, W.,Chen, M.,Yang, Y.,Hu, Y.,Liu, B. Structural basis of copper-efflux-regulator-dependent transcription activation. Iscience, 24:102449-102449, 2021 Cited by PubMed Abstract: The copper efflux regulator (CueR), a representative member of mercury resistance regulator (MerR) family metalloregulators, controls expression of copper homeostasis-regulating genes in bacteria. The mechanism of transcription activation by CueR and other MerR family regulators is bending the spacer domain of promoter DNA. Here, we report the cryo-EM structures of the intact CueR-dependent transcription activation complexes. The structures show that CueR dimer bends the 19-bp promoter spacer to realign the -35 and -10 elements for recognition by σ-RNA polymerase holoenzyme and reveal a previously unreported interaction between the DNA-binding domain (DBD) from one CueR subunit and the σ nonconserved region (σNCR). Functional studies have shown that the CueR-σNCR interaction plays an auxiliary role in CueR-dependent transcription, assisting the activation mechanism of bending promoter DNA by CueR dimer. Because DBDs are highly conserved in sequence and structure, this transcription-activating mechanism could be generally used by MerR family regulators. PubMed: 34113812DOI: 10.1016/j.isci.2021.102449 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.1 Å) |
Structure validation
Download full validation report
