6XH8

CueR-transcription activation complex with RNA transcript

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor RpoD	polymer	628	72206.3	1	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Escherichia coli	Sigma-70
6	G, H (G, H)	HTH-type transcriptional regulator CueR	polymer	143	16327.3	2	UniProt (P0A9G4) Pfam (PF00376) Pfam (PF09278)	Escherichia coli	Copper efflux regulator,Copper export regulator
7	I (1)	NONTEMPLATE STRAND DNA (54-MER)	polymer	54	16604.6	1		Escherichia coli
8	J (2)	TEMPLATE STRAND DNA (54-MER)	polymer	54	16747.7	1		Escherichia coli
9	K (3)	NASCENT RNA	polymer	3	1134.6	1		Escherichia coli
10	L, M (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	N, O (G, H)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)

Sequence modifications

F: 1 - 613 (UniProt: P00579)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Thr -4	-	expression tag
Asp -3	-	expression tag
Gln -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

G, H: 1 - 135 (UniProt: P0A9G4)

PDB	External Database	Details
Leu 136	-	expression tag
Glu 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	528902.4
Non-Polymers*	Number of molecules	4
	Total formula weight	257.9
All*	Total formula weight	529160.3

*Water molecules are not included.