6XF9
Crystal structure of KSHV ORF68
Summary for 6XF9
| Entry DOI | 10.2210/pdb6xf9/pdb |
| Descriptor | Packaging protein UL32, ZINC ION (3 entities in total) |
| Functional Keywords | viral packaging, viral cleavage, viral protein |
| Biological source | Human herpesvirus 8 type M |
| Total number of polymer chains | 5 |
| Total formula weight | 260821.02 |
| Authors | Didychuk, A.L.,Gates, S.N.,Martin, A.,Glaunsinger, B. (deposition date: 2020-06-15, release date: 2021-02-24, Last modification date: 2024-04-03) |
| Primary citation | Didychuk, A.L.,Gates, S.N.,Gardner, M.R.,Strong, L.M.,Martin, A.,Glaunsinger, B.A. A pentameric protein ring with novel architecture is required for herpesviral packaging. Elife, 10:-, 2021 Cited by PubMed Abstract: Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi's sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein-Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor. PubMed: 33554858DOI: 10.7554/eLife.62261 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.22 Å) |
Structure validation
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