6XF9
Crystal structure of KSHV ORF68
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.11589 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 134.987, 224.042, 192.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.540 - 2.220 |
R-factor | 0.2227 |
Rwork | 0.222 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ORF68-polyA |
RMSD bond length | 0.013 |
RMSD bond angle | 1.380 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.543 | 2.480 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 189934 | 4570 |
<I/σ(I)> | 19.4 | 1.7 |
Completeness [%] | 95.3 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 310 mM CaCl2, 95 mM HEPES pH 7.5, 26.6% PEG-400, 5% glycerol |