6X94

An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli

Summary for 6X94

• Entry DOI: 10.2210/pdb6x94/pdb
• Descriptor: Serine--tRNA ligase, 5'-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE, DI(HYDROXYETHYL)ETHER, ... (8 entities in total)
• Functional Keywords: serine trna-ligase, protein translation, serine aminoacylation, non-hydrolyzable serine-amp, ligase
• Biological source: Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)
• Total number of polymer chains: 1
• Total formula weight: 49854.21

Authors

Zambaldo, C.,Koh, M.,Nasertorabi, F.,Han, G.W.,Chatterjee, A.,Stevens, R.C.,Schultz, P.G. (deposition date: 2020-06-02, release date: 2020-11-04, Last modification date: 2023-10-18)

Primary citation

Zambaldo, C.,Koh, M.,Nasertorabi, F.,Han, G.W.,Chatterjee, A.,Stevens, R.C.,Schultz, P.G.
An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli.
Bioorg.Med.Chem., 28:115662-115662, 2020

PubMed Abstract: We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNA)/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).

PubMed: 33069069
DOI: 10.1016/j.bmc.2020.115662

Experimental method

X-RAY DIFFRACTION (1.45 Å)