6X94
An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Serine--tRNA ligase | polymer | 426 | 48659.4 | 1 | UniProt (Q8PYJ6) | Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) | Seryl-tRNA synthetase,SerRS,Seryl-tRNA(Ser/Sec) synthetase |
| 2 | B (A) | 5'-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE | non-polymer | 433.4 | 1 | Chemie (SSA) PubChem (46936812) | |||
| 3 | C, D, E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) PubChem (8117) | |||
| 4 | F (A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 1 | Chemie (DMS) PubChem (679) | |||
| 5 | G, H, I, J, K (A) | THIOCYANATE ION | non-polymer | 58.1 | 5 | Chemie (SCN) PubChem (9322) | |||
| 6 | L (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) PubChem (813) | |||
| 7 | M (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 8 | N (A) | water | water | 18.0 | 683 | Chemie (HOH) |
Sequence modifications
A: 1 - 420 (UniProt: Q8PYJ6)
| PDB | External Database | Details |
|---|---|---|
| Val 402 | Glu 402 | engineered mutation |
| Val 416 | Glu 416 | engineered mutation |
| Pro 419 | Lys 419 | engineered mutation |
| Glu 420 | Val 420 | engineered mutation |
| His 421 | - | expression tag |
| His 422 | - | expression tag |
| His 423 | - | expression tag |
| His 424 | - | expression tag |
| His 425 | - | expression tag |
| His 426 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 48659.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1194.9 | |
| All* | Total formula weight | 49854.2 |
