6WVE
Chicken SPCS1
Summary for 6WVE
| Entry DOI | 10.2210/pdb6wve/pdb |
| Descriptor | Green fluorescent protein,Chicken Signal Peptidase Complex Subunit 1,Green fluorescent protein (2 entities in total) |
| Functional Keywords | signal peptidase complex subunit 1(spcs1), membrane protein |
| Biological source | Aequorea victoria (Jellyfish) More |
| Total number of polymer chains | 1 |
| Total formula weight | 35606.41 |
| Authors | |
| Primary citation | Liu, S.,Li, S.,Yang, Y.,Li, W. Termini restraining of small membrane proteins enables structure determination at near-atomic resolution. Sci Adv, 6:-, 2020 Cited by PubMed Abstract: Small membrane proteins are difficult targets for structural characterization. Here, we stabilize their folding by restraining their amino and carboxyl termini with associable protein entities, exemplified by the two halves of a superfolder GFP. The termini-restrained proteins are functional and show improved stability during overexpression and purification. The reassembled GFP provides a versatile scaffold for membrane protein crystallization, enables diffraction to atomic resolution, and facilitates crystal identification, phase determination, and density modification. This strategy gives rise to 14 new structures of five vertebrate proteins from distinct functional families, bringing a substantial expansion to the structural database of small membrane proteins. Moreover, a high-resolution structure of bacterial DsbB reveals that this thiol oxidoreductase is activated through a catalytic triad, similar to cysteine proteases. Overall, termini restraining proves exceptionally effective for stabilization and structure determination of small membrane proteins. PubMed: 33355146DOI: 10.1126/sciadv.abe3717 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.429 Å) |
Structure validation
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