6WVE
Chicken SPCS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.960, 55.300, 92.720 |
| Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
| Resolution | 49.024 - 2.429 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.429 | 6.650 | 2.450 |
| Rmerge | 0.121 | 0.051 | 1.139 |
| Rmeas | 0.143 | 0.060 | 1.358 |
| Rpim | 0.075 | 0.030 | 0.725 |
| Number of reflections | 18471 | 971 | 932 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 98.0 | 97.8 | 98.4 |
| Redundancy | 3.6 | 3.5 | 3.4 |
| CC(1/2) | 0.998 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 295 | 25% PEG 400, 100 mM ammonium acetate, 0.1 M MES pH 6.0 |
