6WG5
Human ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4, NTPDase 4)
Summary for 6WG5
| Entry DOI | 10.2210/pdb6wg5/pdb |
| Descriptor | Ectonucleoside triphosphate diphosphohydrolase 4, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | nucleotide metabolism, glycosylation, askha superfamily, hydrolase |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 59341.85 |
| Authors | Gorelik, A.,Labriola, J.M.,Illes, K.,Nagar, B. (deposition date: 2020-04-04, release date: 2020-08-12, Last modification date: 2024-10-23) |
| Primary citation | Gorelik, A.,Labriola, J.M.,Illes, K.,Nagar, B. Crystal structure of the nucleotide-metabolizing enzyme NTPDase4. Protein Sci., 29:2054-2061, 2020 Cited by PubMed Abstract: The ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) are a family of enzymes found on the cell surface and in the lumen of certain organelles, that are major regulators of purinergic signaling. Their intracellular roles, however, have not been clearly defined. NTPDase4 (UDPase, ENTPD4) is a Golgi protein potentially involved in nucleotide recycling as part of protein glycosylation, and is also found in lysosomes, where its purpose is unknown. To further our understanding of NTPDase4 function, we determined its crystal structure. The enzyme adopts a wide open, inactive conformation. Differences in the nucleotide-binding site relative to its homologs could account for its substrate selectivity. The putative membrane-interacting loop of cell-surface NTPDases is drastically altered in NTPDase4, potentially affecting its interdomain dynamics at the Golgi membrane. PubMed: 32767432DOI: 10.1002/pro.3926 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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