6WG5
Human ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4, NTPDase 4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-05 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97959 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.378, 101.820, 129.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.322 - 2.600 |
R-factor | 0.1774 |
Rwork | 0.174 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zx3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.521 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.108 | |
Number of reflections | 22486 | 1864 |
<I/σ(I)> | 18.5 | 1.5 |
Completeness [%] | 95.0 | |
Redundancy | 7.2 | |
CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Na/K tartrate, 20% PEG 3350, cryo-protected with 20% glycerol |