6WA8

Crystal structure of the E. coli transcription termination factor Rho

Summary for 6WA8

• Entry DOI: 10.2210/pdb6wa8/pdb
• Descriptor: Transcription termination factor Rho, ADENOSINE-5'-TRIPHOSPHATE (2 entities in total)
• Functional Keywords: atpase, transcription, hydrolase
• Biological source: Escherichia coli (strain K12)
• Total number of polymer chains: 6
• Total formula weight: 285464.09

Authors

Fan, C.,Rees, D.C. (deposition date: 2020-03-24, release date: 2020-09-16, Last modification date: 2023-10-18)

Primary citation

Fan, C.,Rees, D.C.
Crystal structure of the Escherichia coli transcription termination factor Rho.
Acta Crystallogr.,Sect.F, 76:398-405, 2020

PubMed Abstract: During the crystal structure analysis of an ATP-binding cassette (ABC) transporter overexpressed in Escherichia coli, a contaminant protein was crystallized. The identity of the contaminant was revealed by mass spectrometry to be the Escherichia coli transcription terminator factor Rho, structures of which had been previously determined in different conformational states. Although Rho was present at only ∼1% of the target protein (a bacterial homolog of the eukaryotic ABC transporter of mitochondria from Novosphingobium aromaticivorans; NaAtm1), it preferentially crystallized in space group C2 as thin plates that diffracted to 3.30 Å resolution. The structure of Rho in this crystal form exhibits a hexameric open-ring staircase conformation with bound ATP; this characteristic structure was also observed on electron-microscopy grids of the NaAtm1 preparation.

PubMed: 32880587
DOI: 10.1107/S2053230X20010572

Experimental method

X-RAY DIFFRACTION (3.3 Å)