6W9G

Crystal Structure of the Fab fragment of humanized 5c8 antibody containing the fluorescent non-canonical amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine in complex with CD40L at pH 6.8

Summary for 6W9G

• Entry DOI: 10.2210/pdb6w9g/pdb
• Related: 1I9R 6BJZ 6W4W 6W5A
• Descriptor: CD40 ligand, 5c8* Fab (heavy chain), 5c8* Fab (light chain), ... (10 entities in total)
• Functional Keywords: immunoglobulin, cytokine, l-(7-hydroxycoumarin-4-yl)ethylglycine, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 9
• Total formula weight: 198468.31

Authors

Henderson, J.N.,Simmons, C.R.,Mills, J.H. (deposition date: 2020-03-23, release date: 2020-12-23, Last modification date: 2023-11-15)

• Primary citation: Henderson, J.N.,Simmons, C.R.,Fahmi, N.E.,Jeffs, J.W.,Borges, C.R.,Mills, J.H.; Structural Insights into How Protein Environments Tune the Spectroscopic Properties of a Noncanonical Amino Acid Fluorophore.; Biochemistry, 59:3401-3410, 2020; PubMed: 32845612; DOI: 10.1021/acs.biochem.0c00474

Experimental method

X-RAY DIFFRACTION (1.82 Å)