6VV5
Cryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike protein
Summary for 6VV5
| Entry DOI | 10.2210/pdb6vv5/pdb |
| EMDB information | 21391 |
| Descriptor | Spike glycoprotein, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total) |
| Functional Keywords | glycoprotein, surface, receptor-binding, membrane fusion, viral protein |
| Biological source | Porcine epidemic diarrhea virus |
| Total number of polymer chains | 3 |
| Total formula weight | 466495.68 |
| Authors | Kirchdoerfer, R.N.,Ward, A.B. (deposition date: 2020-02-17, release date: 2020-02-26, Last modification date: 2021-04-14) |
| Primary citation | Kirchdoerfer, R.N.,Bhandari, M.,Martini, O.,Sewall, L.M.,Bangaru, S.,Yoon, K.J.,Ward, A.B. Structure and immune recognition of the porcine epidemic diarrhea virus spike protein. Structure, 29:385-392.e5, 2021 Cited by PubMed Abstract: Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a key target for the host antibody response and a good candidate for a protein-based vaccine immunogen. We used electron microscopy to evaluate the PEDV spike structure, as well as pig polyclonal antibody responses to viral infection. The structure of the PEDV spike reveals a configuration similar to that of HuCoV-NL63. Several PEDV protein-protein interfaces are mediated by non-protein components, including a glycan at Asn264 and two bound palmitoleic acid molecules. The polyclonal antibody response to PEDV infection shows a dominance of epitopes in the S1 region. This structural and immune characterization provides insights into coronavirus spike stability determinants and explores the immune landscape of viral spike proteins. PubMed: 33378641DOI: 10.1016/j.str.2020.12.003 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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