6VJE

Crystal structure of Pseudomonas aeruginosa penicillin-binding protein 3 (PBP3) complexed with ceftobiprole

Summary for 6VJE

• Entry DOI: 10.2210/pdb6vje/pdb
• Descriptor: Peptidoglycan D,D-transpeptidase FtsI, (2R)-2-[(1R)-1-{[(2Z)-2-(5-amino-1,2,4-thiadiazol-3-yl)-2-(hydroxyimino)acetyl]amino}-2-oxoethyl]-5-({2-oxo-1-[(3R)-pyr rolidin-3-yl]-2,5-dihydro-1H-pyrrol-3-yl}methyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: antibiotic target, complex, hydrolase
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 1
• Total formula weight: 58936.94

Authors

van den Akker, F.,Kumar, V. (deposition date: 2020-01-15, release date: 2020-03-11, Last modification date: 2024-10-16)

Primary citation

Kumar, V.,Tang, C.,Bethel, C.R.,Papp-Wallace, K.M.,Wyatt, J.,Desarbre, E.,Bonomo, R.A.,van den Akker, F.
Structural Insights into Ceftobiprole Inhibition of Pseudomonas aeruginosa Penicillin-Binding Protein 3.
Antimicrob.Agents Chemother., 64:-, 2020

PubMed Abstract: Ceftobiprole is an advanced-generation broad-spectrum cephalosporin antibiotic with potent and rapid bactericidal activity against Gram-positive pathogens, including methicillin-resistant , as well as susceptible Gram-negative pathogens, including sp. pathogens. In the case of , ceftobiprole acts by inhibiting penicillin-binding protein 3 (PBP3). Structural studies were pursued to elucidate the molecular details of this PBP inhibition. The crystal structure of the His-tagged PBP3-ceftobiprole complex revealed a covalent bond between the ligand and the catalytic residue S294. Ceftobiprole binding leads to large active site changes near binding sites for the pyrrolidinone and pyrrolidine rings. The S528 to L536 region adopts a conformation previously not observed in PBP3, including partial unwinding of the α11 helix. These molecular insights can lead to a deeper understanding of β-lactam-PBP interactions that result in major changes in protein structure, as well as suggesting how to fine-tune current inhibitors and to develop novel inhibitors of this PBP.

PubMed: 32152075
DOI: 10.1128/AAC.00106-20

Experimental method

X-RAY DIFFRACTION (1.76 Å)