6VGS

Alpha-ketoisovalerate decarboxylase (KivD) from Lactococcus lactis, thermostable mutant

This is a non-PDB format compatible entry.

Summary for 6VGS

• Entry DOI: 10.2210/pdb6vgs/pdb
• Descriptor: Alpha-keto acid decarboxylase, MAGNESIUM ION, THIAMINE DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: thiamine pyrophosphate, lyase
• Biological source: Lactococcus lactis subsp. lactis
• Total number of polymer chains: 4
• Total formula weight: 254921.26

Authors

Chan, S.,Korman, T.P.,Sawaya, M.R.,Bowie, J.U. (deposition date: 2020-01-08, release date: 2020-08-05, Last modification date: 2023-10-11)

Primary citation

Sherkhanov, S.,Korman, T.P.,Chan, S.,Faham, S.,Liu, H.,Sawaya, M.R.,Hsu, W.T.,Vikram, E.,Cheng, T.,Bowie, J.U.
Isobutanol production freed from biological limits using synthetic biochemistry.
Nat Commun, 11:4292-4292, 2020

PubMed Abstract: Cost competitive conversion of biomass-derived sugars into biofuel will require high yields, high volumetric productivities and high titers. Suitable production parameters are hard to achieve in cell-based systems because of the need to maintain life processes. As a result, next-generation biofuel production in engineered microbes has yet to match the stringent cost targets set by petroleum fuels. Removing the constraints imposed by having to maintain cell viability might facilitate improved production metrics. Here, we report a cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity of 4 g L h, a titer of 275 g L and 95% yield over the course of nearly 5 days. These production metrics exceed even the highly developed ethanol fermentation process. Our results suggest that moving beyond cells has the potential to expand what is possible for bio-based chemical production.

PubMed: 32855421
DOI: 10.1038/s41467-020-18124-1

Experimental method

X-RAY DIFFRACTION (1.8 Å)