6VGS

Alpha-ketoisovalerate decarboxylase (KivD) from Lactococcus lactis, thermostable mutant

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (AAA, BBB, CCC, DDD)	Alpha-keto acid decarboxylase	polymer	568	63280.7	4	UniProt (A0A0B8QZ66) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Lactococcus lactis subsp. lactis	Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes
2	E, G, I, K (AAA, BBB, CCC, DDD)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	F, H, J, L (AAA, BBB, CCC, DDD)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
4	M, N, O, P (AAA, BBB, CCC, DDD)	water	water		18.0	1181	Chemie (HOH)

Sequence modifications

AAA, BBB, CCC, DDD: 1 - 548 (UniProt: A0A0B8QZ66)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 34	Gln 34	engineered mutation
Val 290	Ala 290	engineered mutation
Pro 386	Ser 386	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	253122.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1798.5
All*	Total formula weight	254921.3

*Water molecules are not included.