6UWK

Engineered variant of I-OnuI meganuclease with improved stability and fully altered specificity targeting human chromosome 11 trans integration site

Summary for 6UWK

• Entry DOI: 10.2210/pdb6uwk/pdb
• Descriptor: I-OnuI-e-Therm-hChr11v3, DNA (27-MER), CALCIUM ION, ... (6 entities in total)
• Functional Keywords: meganuclease, homing endonuclease, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 3
• Total formula weight: 51254.34

Authors

Werther, R.,Stoddard, B.L. (deposition date: 2019-11-05, release date: 2019-12-18, Last modification date: 2023-10-11)

• Primary citation: Lambert, A.R.,Hallinan, J.P.,Werther, R.,Glow, D.,Stoddard, B.L.; Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.; Structure, 28:760-775.e8, 2020; PubMed: 32359399; DOI: 10.1016/j.str.2020.04.009

Experimental method

X-RAY DIFFRACTION (2.533 Å)