6USF

CryoEM structure of human alpha4beta2 nicotinic acetylcholine receptor with varenicline in complex with anti-BRIL synthetic antibody BAK5

Summary for 6USF

• Entry DOI: 10.2210/pdb6usf/pdb
• Related: 6CBV
• EMDB information: 20863
• Descriptor: chimera of soluble cytochrome b562 (BRIL) and neuronal acetylcholine receptor subunit alpha-4, Neuronal acetylcholine receptor subunit beta-2, Fab heavy chain, ... (9 entities in total)
• Functional Keywords: acetylcholine receptor, nicotine, varenicline, anti-bril synthetic antibody, membrane protein, membrane protein-immune system complex, membrane protein/immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 9
• Total formula weight: 347972.25

Authors

Alvarez, F.J.D.,Mukherjee, S.,Han, S.,Ammirati, M.,Kossiakoff, A.A. (deposition date: 2019-10-26, release date: 2020-04-29, Last modification date: 2020-07-29)

• Primary citation: Mukherjee, S.,Erramilli, S.K.,Ammirati, M.,Alvarez, F.J.D.,Fennell, K.F.,Purdy, M.D.,Skrobek, B.M.,Radziwon, K.,Coukos, J.,Kang, Y.,Dutka, P.,Gao, X.,Qiu, X.,Yeager, M.,Eric Xu, H.,Han, S.,Kossiakoff, A.A.; Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.; Nat Commun, 11:1598-1598, 2020; PubMed: 32221310; DOI: 10.1038/s41467-020-15363-0

Experimental method

ELECTRON MICROSCOPY (3.87 Å)