6USF
CryoEM structure of human alpha4beta2 nicotinic acetylcholine receptor with varenicline in complex with anti-BRIL synthetic antibody BAK5
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | chimera of soluble cytochrome b562 (BRIL) and neuronal acetylcholine receptor subunit alpha-4 | polymer | 486 | 55914.7 | 2 | UniProt (P43681) UniProt (P0ABE7) Pfam (PF02931) Pfam (PF02932) Pfam (PF07361) In PDB | Homo sapiens (Human) | Cytochrome b-562 |
2 | B, C, E | Neuronal acetylcholine receptor subunit beta-2 | polymer | 397 | 45932.0 | 3 | UniProt (P17787) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (Human) | |
3 | H, K | Fab heavy chain | polymer | 229 | 24351.1 | 2 | Homo sapiens (Human) | ||
4 | L, M | Fab light chain | polymer | 215 | 23483.1 | 2 | Homo sapiens (Human) | ||
5 | F, G | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 383.3 | 2 | In PDB GlyTouCan (G90333CG) | |||
6 | I | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
7 | A, B, C, D | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
8 | A, D | VARENICLINE | non-polymer | 211.3 | 2 | Chemie (QMR) | |||
9 | E | beta-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (BMA) |
Sequence modifications
A, D: 1 - 332 (UniProt: P43681)
A, D: 343 - 443 (UniProt: P0ABE7)
A, D: null - 486 (UniProt: P43681)
B, C, E: 1 - 326 (UniProt: P17787)
B, C, E: 329 - 389 (UniProt: P17787)
A, D: 343 - 443 (UniProt: P0ABE7)
PDB | External Database | Details |
---|---|---|
Trp 349 | Met 29 | conflict |
Ile 444 | - | linker |
Gln 445 | - | linker |
Lys | - | linker |
Tyr | - | linker |
PDB | External Database | Details |
---|---|---|
Lys 333 | - | linker |
Arg 334 | - | linker |
Pro 335 | - | linker |
Ser 336 | - | linker |
Val 337 | - | linker |
Val 338 | - | linker |
Asp 339 | - | linker |
Thr 340 | - | linker |
Asp 341 | - | linker |
Phe 342 | - | linker |
B, C, E: 329 - 389 (UniProt: P17787)
PDB | External Database | Details |
---|---|---|
Ser 390 | - | expression tag |
Ala 391 | - | expression tag |
Trp 392 | - | expression tag |
Ser 393 | - | expression tag |
His 394 | - | expression tag |
Pro 395 | - | expression tag |
Gln 396 | - | expression tag |
Phe 397 | - | expression tag |
Glu 398 | - | expression tag |
Lys 399 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 345293.6 | |
Branched | Number of molecules | 3 |
Total formula weight | 1191.1 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1487.5 | |
All* | Total formula weight | 347972.3 |