6USF
CryoEM structure of human alpha4beta2 nicotinic acetylcholine receptor with varenicline in complex with anti-BRIL synthetic antibody BAK5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | chimera of soluble cytochrome b562 (BRIL) and neuronal acetylcholine receptor subunit alpha-4 | polymer | 486 | 55914.7 | 2 | UniProt (P43681) UniProt (P0ABE7) Pfam (PF02931) Pfam (PF02932) Pfam (PF07361) | Homo sapiens (Human) | Cytochrome b-562 |
| 2 | B, C, E (B, C, E) | Neuronal acetylcholine receptor subunit beta-2 | polymer | 397 | 45932.0 | 3 | UniProt (P17787) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | |
| 3 | F, H (H, K) | Fab heavy chain | polymer | 229 | 24351.1 | 2 | Homo sapiens (Human) | ||
| 4 | G, I (L, M) | Fab light chain | polymer | 215 | 23483.1 | 2 | Homo sapiens (Human) | ||
| 5 | J, K (F, G) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 383.3 | 2 | In PDB GlyTouCan (G90333CG) | |||
| 6 | L (I) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 7 | M, O, P, Q (A, B, C, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 8 | N, R (A, D) | VARENICLINE | non-polymer | 211.3 | 2 | Chemie (QMR) | |||
| 9 | S (E) | beta-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (BMA) |
Sequence modifications
A, D: 1 - 332 (UniProt: P43681)
A, D: 343 - 443 (UniProt: P0ABE7)
A, D: null - 486 (UniProt: P43681)
B, C, E: 1 - 326 (UniProt: P17787)
B, C, E: 329 - 389 (UniProt: P17787)
A, D: 343 - 443 (UniProt: P0ABE7)
| PDB | External Database | Details |
|---|---|---|
| Trp 349 | Met 29 | conflict |
| Ile 444 | - | linker |
| Gln 445 | - | linker |
| Lys | - | linker |
| Tyr | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 333 | - | linker |
| Arg 334 | - | linker |
| Pro 335 | - | linker |
| Ser 336 | - | linker |
| Val 337 | - | linker |
| Val 338 | - | linker |
| Asp 339 | - | linker |
| Thr 340 | - | linker |
| Asp 341 | - | linker |
| Phe 342 | - | linker |
B, C, E: 329 - 389 (UniProt: P17787)
| PDB | External Database | Details |
|---|---|---|
| Ser 390 | - | expression tag |
| Ala 391 | - | expression tag |
| Trp 392 | - | expression tag |
| Ser 393 | - | expression tag |
| His 394 | - | expression tag |
| Pro 395 | - | expression tag |
| Gln 396 | - | expression tag |
| Phe 397 | - | expression tag |
| Glu 398 | - | expression tag |
| Lys 399 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 345293.6 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1191.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1487.5 | |
| All* | Total formula weight | 347972.3 |
