6TXD

Variant W229D/F290W-12 of the last common ancestor of Gram-negative bacteria beta-lactamase class A (GNCA4)

Summary for 6TXD

• Entry DOI: 10.2210/pdb6txd/pdb
• Related: 5FQI 5FQK
• Descriptor: Beta lactamase (GNCA4-12), GLYCEROL, FORMIC ACID, ... (6 entities in total)
• Functional Keywords: hydrolase, antibiotic resistance, ancestral reconstructed
• Biological source: synthetic construct
• Total number of polymer chains: 3
• Total formula weight: 87743.43

Authors

Gavira, J.A.,Risso, V.,Sanchez-Ruiz, J.M.,Romero-Rivera, A.,Kamerlin, S.C.L. (deposition date: 2020-01-14, release date: 2020-06-03, Last modification date: 2024-01-24)

Primary citation

Risso, V.A.,Romero-Rivera, A.,Gutierrez-Rus, L.I.,Ortega-Munoz, M.,Santoyo-Gonzalez, F.,Gavira, J.A.,Sanchez-Ruiz, J.M.,Kamerlin, S.C.L.
Enhancing ade novoenzyme activity by computationally-focused ultra-low-throughput screening.
Chem Sci, 11:6134-6148, 2020

PubMed Abstract: Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic analysis and Rosetta design to rank enzyme variants with multiple mutations, on the basis of predicted stability. Here, we use it to target the active site region of a minimalist-designed, Kemp eliminase. The similarity between the Michaelis complex and transition state for the enzymatic reaction makes this system particularly challenging to optimize. Yet, experimental screening of a small number of active-site variants at the top of the predicted stability ranking leads to catalytic efficiencies and turnover numbers (∼2 × 10 M s and ∼10 s) for this anthropogenic reaction that compare favorably to those of modern natural enzymes. This result illustrates the promise of FuncLib as a powerful tool with which to speed up directed evolution, even on scaffolds that were not originally evolved for those functions, by guiding screening to regions of the sequence space that encode stable and catalytically diverse enzymes. Empirical valence bond calculations reproduce the experimental activation energies for the optimized eliminases to within ∼2 kcal mol and indicate that the enhanced activity is linked to better geometric preorganization of the active site. This raises the possibility of further enhancing the stability-guidance of FuncLib by computational predictions of catalytic activity, as a generalized approach for computational enzyme design.

PubMed: 32832059
DOI: 10.1039/d0sc01935f

Experimental method

X-RAY DIFFRACTION (2 Å)