6TUT

Cryo-EM structure of the RNA Polymerase III-Maf1 complex

Summary for 6TUT

Entry DOI	10.2210/pdb6tut/pdb
EMDB information	10595
Descriptor	DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (19 entities in total)
Functional Keywords	transcription, rna polymerase iii, pol iii, maf1, transcription inhibition
Biological source	Saccharomyces cerevisiae S288C More
Total number of polymer chains	18
Total formula weight	725061.82
Authors	Vorlaender, M.K.,Hagen, W.J.H.,Mueller, C.W. (deposition date: 2020-01-08, release date: 2020-02-19, Last modification date: 2024-05-22)
Primary citation	Vorlander, M.K.,Baudin, F.,Moir, R.D.,Wetzel, R.,Hagen, W.J.H.,Willis, I.M.,Muller, C.W. Structural basis for RNA polymerase III transcription repression by Maf1. Nat.Struct.Mol.Biol., 27:229-232, 2020 Cited by PubMed Abstract: Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex. PubMed: 32066962 DOI: 10.1038/s41594-020-0383-y PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.25 Å)

Structure validation