6TUT

Cryo-EM structure of the RNA Polymerase III-Maf1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase III subunit RPC1	polymer	1460	162517.8	1	UniProt (P04051) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C1,DNA-directed RNA polymerase III largest subunit,RNA polymerase III subunit C160
2	B	DNA-directed RNA polymerase III subunit RPC2	polymer	1149	129629.4	1	UniProt (P22276) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C2,C128,DNA-directed RNA polymerase III 130 kDa polypeptide
3	C	DNA-directed RNA polymerases I and III subunit RPAC1	polymer	335	37732.6	1	UniProt (P07703) Pfam (PF01193) Pfam (PF01000) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I and III subunit AC1,C37,DNA-directed RNA polymerases I and III 40 kDa polypeptide,C40
4	D	DNA-directed RNA polymerase III subunit RPC9	polymer	161	18623.1	1	UniProt (P47076) Pfam (PF03874) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C9,RNA polymerase III subunit C17
5	E	DNA-directed RNA polymerases I, II, and III subunit RPABC1	polymer	215	25117.1	1	UniProt (P20434) Pfam (PF03871) Pfam (PF01191) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC1,ABC27,DNA-directed RNA polymerases I,and III 27 kDa polypeptide
6	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	155	17931.8	1	UniProt (P20435) Pfam (PF01192) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC2,ABC23,DNA-directed RNA polymerases I,and III 23 kDa polypeptide
7	G	DNA-directed RNA polymerase III subunit RPC8	polymer	212	24349.8	1	UniProt (P35718) Pfam (PF03876) Pfam (PF08292) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C8,DNA-directed RNA polymerase III 25 kDa polypeptide,RNA polymerase III subunit C25
8	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	146	16525.4	1	UniProt (P20436) Pfam (PF03870) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC3,ABC14.4,ABC14.5,DNA-directed RNA polymerases I,and III 14.5 kDa polypeptide
9	I	DNA-directed RNA polymerase III subunit RPC10	polymer	110	12525.1	1	UniProt (Q04307) Pfam (PF02150) Pfam (PF01096) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C10,DNA-directed RNA polymerases III 12.5 kDa polypeptide,RNA polymerase III subunit C11
10	J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	70	8290.7	1	UniProt (P22139) Pfam (PF01194) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC5,ABC10-beta,ABC8,DNA-directed RNA polymerases I,and III 8.3 kDa polypeptide
11	K	DNA-directed RNA polymerases I and III subunit RPAC2	polymer	142	16167.9	1	UniProt (P28000) Pfam (PF13656) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I and III subunit AC2,AC19,DNA-directed RNA polymerases I and III 16 kDa polypeptide,RPA19
12	L	DNA-directed RNA polymerases I, II, and III subunit RPABC4	polymer	70	7730.0	1	UniProt (P40422) Pfam (PF03604) In PDB	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC4,ABC10-alpha
13	M	DNA-directed RNA polymerase III subunit RPC5	polymer	282	32178.1	1	UniProt (P36121) Pfam (PF04801) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C5,DNA-directed RNA polymerase III 37 kDa polypeptide,RNA polymerase III subunit C37
14	N	DNA-directed RNA polymerase III subunit RPC4	polymer	422	46751.5	1	UniProt (P25441) Pfam (PF05132) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C4,C53,DNA-directed RNA polymerase III 47 kDa polypeptide
15	O	DNA-directed RNA polymerase III subunit RPC3	polymer	654	74112.8	1	UniProt (P32349) Pfam (PF08221) Pfam (PF05645) Pfam (PF22536) Pfam (PF20912) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C3,C82,DNA-directed III 74 kDa polypeptide,C74
16	P	DNA-directed RNA polymerase III subunit RPC6	polymer	317	36174.2	1	UniProt (P32910) Pfam (PF05158) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C6,C34,DNA-directed RNA polymerase III 36 kDa polypeptide
17	Q	DNA-directed RNA polymerase III subunit RPC7	polymer	251	27753.0	1	UniProt (P17890) Pfam (PF11705) In PDB	Saccharomyces cerevisiae S288C	RNA polymerase III subunit C7,DNA-directed RNA polymerase III 31 kDa polypeptide,C31
18	R	Repressor of RNA polymerase III transcription MAF1,Repressor of RNA polymerase III transcription MAF1	polymer	267	30493.8	1	UniProt (P41910) Pfam (PF09174) In PDB	Saccharomyces cerevisiae S288C
19	A, B, I, J, L	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Sequence modifications

R: 2 - 194 (UniProt: P41910)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag

R: 195 - 395 (UniProt: P41910)

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	724604.0
Non-Polymers*	Number of molecules	7
	Total formula weight	457.9
All*	Total formula weight	725061.8

*Water molecules are not included.