6TUT
Cryo-EM structure of the RNA Polymerase III-Maf1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase III subunit RPC1 | polymer | 1460 | 162517.8 | 1 | UniProt (P04051) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C1,DNA-directed RNA polymerase III largest subunit,RNA polymerase III subunit C160 |
| 2 | B (B) | DNA-directed RNA polymerase III subunit RPC2 | polymer | 1149 | 129629.4 | 1 | UniProt (P22276) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C2,C128,DNA-directed RNA polymerase III 130 kDa polypeptide |
| 3 | C (C) | DNA-directed RNA polymerases I and III subunit RPAC1 | polymer | 335 | 37732.6 | 1 | UniProt (P07703) Pfam (PF01193) Pfam (PF01000) | Saccharomyces cerevisiae S288C | RNA polymerases I and III subunit AC1,C37,DNA-directed RNA polymerases I and III 40 kDa polypeptide,C40 |
| 4 | D (D) | DNA-directed RNA polymerase III subunit RPC9 | polymer | 161 | 18623.1 | 1 | UniProt (P47076) Pfam (PF03874) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C9,RNA polymerase III subunit C17 |
| 5 | E (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 215 | 25117.1 | 1 | UniProt (P20434) Pfam (PF03871) Pfam (PF01191) | Saccharomyces cerevisiae S288C | RNA polymerases I,II,and III subunit ABC1,ABC27,DNA-directed RNA polymerases I,and III 27 kDa polypeptide |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 155 | 17931.8 | 1 | UniProt (P20435) Pfam (PF01192) | Saccharomyces cerevisiae S288C | RNA polymerases I,II,and III subunit ABC2,ABC23,DNA-directed RNA polymerases I,and III 23 kDa polypeptide |
| 7 | G (G) | DNA-directed RNA polymerase III subunit RPC8 | polymer | 212 | 24349.8 | 1 | UniProt (P35718) Pfam (PF03876) Pfam (PF08292) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C8,DNA-directed RNA polymerase III 25 kDa polypeptide,RNA polymerase III subunit C25 |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 146 | 16525.4 | 1 | UniProt (P20436) Pfam (PF03870) | Saccharomyces cerevisiae S288C | RNA polymerases I,II,and III subunit ABC3,ABC14.4,ABC14.5,DNA-directed RNA polymerases I,and III 14.5 kDa polypeptide |
| 9 | I (I) | DNA-directed RNA polymerase III subunit RPC10 | polymer | 110 | 12525.1 | 1 | UniProt (Q04307) Pfam (PF02150) Pfam (PF01096) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C10,DNA-directed RNA polymerases III 12.5 kDa polypeptide,RNA polymerase III subunit C11 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 70 | 8290.7 | 1 | UniProt (P22139) Pfam (PF01194) | Saccharomyces cerevisiae S288C | RNA polymerases I,II,and III subunit ABC5,ABC10-beta,ABC8,DNA-directed RNA polymerases I,and III 8.3 kDa polypeptide |
| 11 | K (K) | DNA-directed RNA polymerases I and III subunit RPAC2 | polymer | 142 | 16167.9 | 1 | UniProt (P28000) Pfam (PF13656) | Saccharomyces cerevisiae S288C | RNA polymerases I and III subunit AC2,AC19,DNA-directed RNA polymerases I and III 16 kDa polypeptide,RPA19 |
| 12 | L (L) | DNA-directed RNA polymerases I, II, and III subunit RPABC4 | polymer | 70 | 7730.0 | 1 | UniProt (P40422) Pfam (PF03604) | Saccharomyces cerevisiae S288C | RNA polymerases I,II,and III subunit ABC4,ABC10-alpha |
| 13 | M (M) | DNA-directed RNA polymerase III subunit RPC5 | polymer | 282 | 32178.1 | 1 | UniProt (P36121) Pfam (PF04801) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C5,DNA-directed RNA polymerase III 37 kDa polypeptide,RNA polymerase III subunit C37 |
| 14 | N (N) | DNA-directed RNA polymerase III subunit RPC4 | polymer | 422 | 46751.5 | 1 | UniProt (P25441) Pfam (PF05132) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C4,C53,DNA-directed RNA polymerase III 47 kDa polypeptide |
| 15 | O (O) | DNA-directed RNA polymerase III subunit RPC3 | polymer | 654 | 74112.8 | 1 | UniProt (P32349) Pfam (PF08221) Pfam (PF05645) Pfam (PF22536) Pfam (PF20912) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C3,C82,DNA-directed III 74 kDa polypeptide,C74 |
| 16 | P (P) | DNA-directed RNA polymerase III subunit RPC6 | polymer | 317 | 36174.2 | 1 | UniProt (P32910) Pfam (PF05158) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C6,C34,DNA-directed RNA polymerase III 36 kDa polypeptide |
| 17 | Q (Q) | DNA-directed RNA polymerase III subunit RPC7 | polymer | 251 | 27753.0 | 1 | UniProt (P17890) Pfam (PF11705) | Saccharomyces cerevisiae S288C | RNA polymerase III subunit C7,DNA-directed RNA polymerase III 31 kDa polypeptide,C31 |
| 18 | R (R) | Repressor of RNA polymerase III transcription MAF1,Repressor of RNA polymerase III transcription MAF1 | polymer | 267 | 30493.8 | 1 | UniProt (P41910) Pfam (PF09174) | Saccharomyces cerevisiae S288C | |
| 19 | S, T, U, V, W... (A, B, I, J, L) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) |
Sequence modifications
R: 2 - 194 (UniProt: P41910)
R: 195 - 395 (UniProt: P41910)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 724604.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 457.9 | |
| All* | Total formula weight | 725061.8 |
