6T21

N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5mCGA target sequence

Summary for 6T21

• Entry DOI: 10.2210/pdb6t21/pdb
• Related: 6GHC 6R64 6T22
• Descriptor: 5-methylcytosine-specific restriction enzyme A, DNA (5'-D(*TP*CP*AP*TP*(5CM)P*GP*AP*TP*TP*C)-3'), DNA (5'-D(*GP*AP*AP*TP*(5CM)P*GP*AP*TP*GP*A)-3'), ... (4 entities in total)
• Functional Keywords: ecokmcra, neco, n-terminal domain, modification dependent restriction, 5-methylcytosine, 5mc, 5-hydroxymethylcytosine, 5hmc, hnh endonuclease, bba-me nuclease, hydrolase
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 6
• Total formula weight: 46969.38

Authors

Slyvka, A.,Zagorskaite, E.,Czapinska, H.,Sasnauskas, G.,Bochtler, M. (deposition date: 2019-10-07, release date: 2019-10-23, Last modification date: 2024-01-24)

• Primary citation: Slyvka, A.,Zagorskaite, E.,Czapinska, H.,Sasnauskas, G.,Bochtler, M.; Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping.; Nucleic Acids Res., 47:11943-11955, 2019; PubMed: 31724709; DOI: 10.1093/nar/gkz1017

Experimental method

X-RAY DIFFRACTION (2.07 Å)