6T21
N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5mCGA target sequence
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | 5-methylcytosine-specific restriction enzyme A | polymer | 152 | 17368.6 | 2 | UniProt (P24200) In PDB | Escherichia coli (strain K12) | EcoKMcrA |
2 | C, E | DNA (5'-D(*TP*CP*AP*TP*(5CM)P*GP*AP*TP*TP*C)-3') | polymer | 10 | 3009.0 | 2 | synthetic construct | ||
3 | D, F | DNA (5'-D(*GP*AP*AP*TP*(5CM)P*GP*AP*TP*GP*A)-3') | polymer | 10 | 3107.1 | 2 | synthetic construct | ||
4 | water | water | 18.0 | 431 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 143 (UniProt: P24200)
PDB | External Database | Details |
---|---|---|
Gly -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
Glu -1 | - | expression tag |
Phe 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 46969.4 | |
All* | Total formula weight | 46969.4 |