6SZA
MoxR AAA-ATPase RavA, C2-symmetric closed ring conformation
Summary for 6SZA
Entry DOI | 10.2210/pdb6sza/pdb |
Related | 6Q7L 6Q7M 6SZB |
EMDB information | 10351 |
Descriptor | RavA, ADENOSINE-5'-DIPHOSPHATE (2 entities in total) |
Functional Keywords | aaa+ atpase, moxr, escherichia coli, chaperone |
Biological source | Escherichia coli K-12 |
Total number of polymer chains | 6 |
Total formula weight | 340436.32 |
Authors | Jessop, M.,Felix, J.,Gutsche, I. (deposition date: 2019-10-02, release date: 2020-02-19, Last modification date: 2024-05-22) |
Primary citation | Jessop, M.,Arragain, B.,Miras, R.,Fraudeau, A.,Huard, K.,Bacia-Verloop, M.,Catty, P.,Felix, J.,Malet, H.,Gutsche, I. Structural insights into ATP hydrolysis by the MoxR ATPase RavA and the LdcI-RavA cage-like complex. Commun Biol, 3:46-46, 2020 Cited by PubMed Abstract: The hexameric MoxR AAA+ ATPase RavA and the decameric lysine decarboxylase LdcI form a 3.3 MDa cage, proposed to assist assembly of specific respiratory complexes in E. coli. Here, we show that inside the LdcI-RavA cage, RavA hexamers adopt an asymmetric spiral conformation in which the nucleotide-free seam is constrained to two opposite orientations. Cryo-EM reconstructions of free RavA reveal two co-existing structural states: an asymmetric spiral, and a flat C2-symmetric closed ring characterised by two nucleotide-free seams. The closed ring RavA state bears close structural similarity to the pseudo two-fold symmetric crystal structure of the AAA+ unfoldase ClpX, suggesting a common ATPase mechanism. Based on these structures, and in light of the current knowledge regarding AAA+ ATPases, we propose different scenarios for the ATP hydrolysis cycle of free RavA and the LdcI-RavA cage-like complex, and extend the comparison to other AAA+ ATPases of clade 7. PubMed: 31992852DOI: 10.1038/s42003-020-0772-0 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (6 Å) |
Structure validation
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