6Q7M
Spiral structure of E. coli RavA in the RavA-LdcI cage-like complex
This is a non-PDB format compatible entry.
Summary for 6Q7M
Entry DOI | 10.2210/pdb6q7m/pdb |
EMDB information | 2679 4469 4470 |
Descriptor | Inducible lysine decarboxylase, ATPase RavA, PYRIDOXAL-5'-PHOSPHATE, ... (4 entities in total) |
Functional Keywords | complex, moxr atpase, lysine decarboxylase, hydrolase |
Biological source | Escherichia coli More |
Total number of polymer chains | 26 |
Total formula weight | 1972327.68 |
Authors | Arragain, B.,Felix, J.,Malet, H.,Gutsche, I.,Jessop, M. (deposition date: 2018-12-13, release date: 2020-02-12, Last modification date: 2025-04-09) |
Primary citation | Jessop, M.,Arragain, B.,Miras, R.,Fraudeau, A.,Huard, K.,Bacia-Verloop, M.,Catty, P.,Felix, J.,Malet, H.,Gutsche, I. Structural insights into ATP hydrolysis by the MoxR ATPase RavA and the LdcI-RavA cage-like complex. Commun Biol, 3:46-46, 2020 Cited by PubMed Abstract: The hexameric MoxR AAA+ ATPase RavA and the decameric lysine decarboxylase LdcI form a 3.3 MDa cage, proposed to assist assembly of specific respiratory complexes in E. coli. Here, we show that inside the LdcI-RavA cage, RavA hexamers adopt an asymmetric spiral conformation in which the nucleotide-free seam is constrained to two opposite orientations. Cryo-EM reconstructions of free RavA reveal two co-existing structural states: an asymmetric spiral, and a flat C2-symmetric closed ring characterised by two nucleotide-free seams. The closed ring RavA state bears close structural similarity to the pseudo two-fold symmetric crystal structure of the AAA+ unfoldase ClpX, suggesting a common ATPase mechanism. Based on these structures, and in light of the current knowledge regarding AAA+ ATPases, we propose different scenarios for the ATP hydrolysis cycle of free RavA and the LdcI-RavA cage-like complex, and extend the comparison to other AAA+ ATPases of clade 7. PubMed: 31992852DOI: 10.1038/s42003-020-0772-0 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (7.8 Å) |
Structure validation
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