6SYT
Structure of the SMG1-SMG8-SMG9 complex
Summary for 6SYT
| Entry DOI | 10.2210/pdb6syt/pdb |
| EMDB information | 10347 |
| Descriptor | SMG1,Serine/threonine-protein kinase SMG1,SMG1,Serine/threonine-protein kinase SMG1,SMG1,Serine/threonine-protein kinase SMG1,SMG1,Serine/threonine-protein kinase SMG1,SMG1,Serine/threonine-protein kinase SMG1, Protein SMG8, Protein SMG9, ... (6 entities in total) |
| Functional Keywords | kinase, nmd, ip6, g-fold protein, pikk family, signaling protein |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 3 |
| Total formula weight | 570138.68 |
| Authors | Gat, Y.,Schuller, J.M.,Conti, E. (deposition date: 2019-10-01, release date: 2019-12-11, Last modification date: 2024-05-22) |
| Primary citation | Gat, Y.,Schuller, J.M.,Lingaraju, M.,Weyher, E.,Bonneau, F.,Strauss, M.,Murray, P.J.,Conti, E. InsP6binding to PIKK kinases revealed by the cryo-EM structure of an SMG1-SMG8-SMG9 complex. Nat.Struct.Mol.Biol., 26:1089-1093, 2019 Cited by PubMed Abstract: We report the 3.45-Å resolution cryo-EM structure of human SMG1-SMG8-SMG9, a phosphatidylinositol-3-kinase (PI(3)K)-related protein kinase (PIKK) complex central to messenger RNA surveillance. Structural and MS analyses reveal the presence of inositol hexaphosphate (InsP) in the SMG1 kinase. We show that the InsP-binding site is conserved in mammalian target of rapamycin (mTOR) and potentially other PIKK members, and that it is required for optimal in vitro phosphorylation of both SMG1 and mTOR substrates. PubMed: 31792449DOI: 10.1038/s41594-019-0342-7 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.45 Å) |
Structure validation
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