6SN1

Crystal structure of the human INTS13-INTS14 complex

Summary for 6SN1

• Entry DOI: 10.2210/pdb6sn1/pdb
• Descriptor: Integrator complex subunit 13, Integrator complex subunit 14, SULFATE ION, ... (4 entities in total)
• Functional Keywords: integrator, interlocked chains, vwa, pseudosymmetrical heterodimer, rna binding protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 144172.24

Authors

Jonas, S.,Sabath, K.,Staeubli, M.L. (deposition date: 2019-08-23, release date: 2020-07-22, Last modification date: 2024-05-15)

Primary citation

Sabath, K.,Staubli, M.L.,Marti, S.,Leitner, A.,Moes, M.,Jonas, S.
INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.
Nat Commun, 11:3422-3422, 2020

PubMed Abstract: The Integrator complex processes 3'-ends of spliceosomal small nuclear RNAs (snRNAs). Furthermore, it regulates transcription of protein coding genes by terminating transcription after unstable pausing. The molecular basis for Integrator's functions remains obscure. Here, we show that INTS10, Asunder/INTS13 and INTS14 form a separable, functional Integrator module. The structure of INTS13-INTS14 reveals a strongly entwined complex with a unique chain interlink. Unexpected structural homology to the Ku70-Ku80 DNA repair complex suggests nucleic acid affinity. Indeed, the module displays affinity for DNA and RNA but prefers RNA hairpins. While the module plays an accessory role in snRNA maturation, it has a stronger influence on transcription termination after pausing. Asunder/INTS13 directly binds Integrator's cleavage module via a conserved C-terminal motif that is involved in snRNA processing and required for spermatogenesis. Collectively, our data establish INTS10-INTS13-INTS14 as a nucleic acid-binding module and suggest that it brings cleavage module and target transcripts into proximity.

PubMed: 32647223
DOI: 10.1038/s41467-020-17232-2

Experimental method

X-RAY DIFFRACTION (2.54 Å)