6SN1

Crystal structure of the human INTS13-INTS14 complex

6SN1 の概要

• エントリーDOI: 10.2210/pdb6sn1/pdb
• 分子名称: Integrator complex subunit 13, Integrator complex subunit 14, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: integrator, interlocked chains, vwa, pseudosymmetrical heterodimer, rna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 144172.24

構造登録者

Jonas, S.,Sabath, K.,Staeubli, M.L. (登録日: 2019-08-23, 公開日: 2020-07-22, 最終更新日: 2024-05-15)

主引用文献

Sabath, K.,Staubli, M.L.,Marti, S.,Leitner, A.,Moes, M.,Jonas, S.
INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.
Nat Commun, 11:3422-3422, 2020

PubMed Abstract: The Integrator complex processes 3'-ends of spliceosomal small nuclear RNAs (snRNAs). Furthermore, it regulates transcription of protein coding genes by terminating transcription after unstable pausing. The molecular basis for Integrator's functions remains obscure. Here, we show that INTS10, Asunder/INTS13 and INTS14 form a separable, functional Integrator module. The structure of INTS13-INTS14 reveals a strongly entwined complex with a unique chain interlink. Unexpected structural homology to the Ku70-Ku80 DNA repair complex suggests nucleic acid affinity. Indeed, the module displays affinity for DNA and RNA but prefers RNA hairpins. While the module plays an accessory role in snRNA maturation, it has a stronger influence on transcription termination after pausing. Asunder/INTS13 directly binds Integrator's cleavage module via a conserved C-terminal motif that is involved in snRNA processing and required for spermatogenesis. Collectively, our data establish INTS10-INTS13-INTS14 as a nucleic acid-binding module and suggest that it brings cleavage module and target transcripts into proximity.

PubMed: 32647223
DOI: 10.1038/s41467-020-17232-2

実験手法

X-RAY DIFFRACTION (2.54 Å)