6SGB

mt-SSU assemblosome of Trypanosoma brucei

This is a non-PDB format compatible entry.

Summary for 6SGB

• Entry DOI: 10.2210/pdb6sgb/pdb
• EMDB information: 10180
• Descriptor: uS5m, mS23, UNK-i, ... (110 entities in total)
• Functional Keywords: mitochondrial ribosome, assembly intermediate, translation, ribosome
• Biological source: Trypanosoma brucei brucei; More
• Total number of polymer chains: 116
• Total formula weight: 4340629.68

Authors

Saurer, M.,Ramrath, D.J.F.,Niemann, M.,Calderaro, S.,Prange, C.,Mattei, S.,Scaiola, A.,Leitner, A.,Bieri, P.,Horn, E.K.,Leibundgut, M.,Boehringer, D.,Schneider, A.,Ban, N. (deposition date: 2019-08-03, release date: 2019-09-25, Last modification date: 2025-12-10)

Primary citation

Saurer, M.,Ramrath, D.J.F.,Niemann, M.,Calderaro, S.,Prange, C.,Mattei, S.,Scaiola, A.,Leitner, A.,Bieri, P.,Horn, E.K.,Leibundgut, M.,Boehringer, D.,Schneider, A.,Ban, N.
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Science, 365:1144-1149, 2019

PubMed Abstract: Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.

PubMed: 31515389
DOI: 10.1126/science.aaw5570

Experimental method

ELECTRON MICROSCOPY (3.3 Å)