6S20

Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33336S-sulf)

6S20 の概要

• エントリーDOI: 10.2210/pdb6s20/pdb
• 分子名称: N-acetylgalactosamine-6-O-sulfatase, 2-acetamido-2-deoxy-6-O-sulfo-beta-D-galactopyranose, TRIETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: gut microbiota, glycosaminoglycan, glycobiology, cazymes, hydrolase
• 由来する生物種: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57126.89

構造登録者

Ndeh, D.,Basle, A.,Strahl, H.,Henrissat, B.,Terrapon, N.,Cartmell, A. (登録日: 2019-06-19, 公開日: 2020-02-05, 最終更新日: 2024-05-15)

主引用文献

Ndeh, D.,Basle, A.,Strahl, H.,Yates, E.A.,McClurgg, U.L.,Henrissat, B.,Terrapon, N.,Cartmell, A.
Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus.
Nat Commun, 11:646-646, 2020

PubMed Abstract: The human gut microbiota (HGM), which is critical to human health, utilises complex glycans as its major carbon source. Glycosaminoglycans represent an important, high priority, nutrient source for the HGM. Pathways for the metabolism of various glycosaminoglycan substrates remain ill-defined. Here we perform a biochemical, genetic and structural dissection of the genetic loci that orchestrates glycosaminoglycan metabolism in the organism Bacteroides thetaiotaomicron. Here, we report: the discovery of two previously unknown surface glycan binding proteins which facilitate glycosaminoglycan import into the periplasm; distinct kinetic and genetic specificities of various periplasmic lyases which dictate glycosaminoglycan metabolic pathways; understanding of endo sulfatase activity questioning the paradigm of how the 'sulfation problem' is handled by the HGM; and 3D crystal structures of the polysaccharide utilisation loci encoded sulfatases. Together with comparative genomic studies, our study fills major gaps in our knowledge of glycosaminoglycan metabolism by the HGM.

PubMed: 32005816
DOI: 10.1038/s41467-020-14509-4

実験手法

X-RAY DIFFRACTION (1.98 Å)