6S20
Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33336S-sulf)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2019-01-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.400, 86.400, 122.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.650 - 1.980 |
R-factor | 0.18249 |
Rwork | 0.180 |
R-free | 0.22364 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.531 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.650 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 32979 | 2253 |
<I/σ(I)> | 11 | |
Completeness [%] | 100.0 | |
Redundancy | 14.2 | |
CC(1/2) | 0.998 | 0.561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30 % PEG 4000, 200 mM MgCl2, and 0.1 M Tris, pH 8.5. 20m/gml protein and 10 mM GlaNAc6S |