6ROW

Haemonchus galactose containing glycoprotein complex

Summary for 6ROW

• Entry DOI: 10.2210/pdb6row/pdb
• EMDB information: 4975
• Descriptor: Putative zinc metallopeptidase, Parasite pepsinogen, Cysteine Protease (3 entities in total)
• Functional Keywords: multi-protease complex, hydrolase
• Biological source: Haemonchus contortus (Barber pole worm); More
• Total number of polymer chains: 7
• Total formula weight: 456215.68

Authors

Scarff, C.A.,Thompson, R.F.,Newlands, G.F.J.,Jamson, H.,Kennaway, C.,da Silva, V.J.,Rabelo, E.M.,Song, C.F.,Trinick, J.,Smith, W.D.,Muench, S.P. (deposition date: 2019-05-13, release date: 2020-03-25, Last modification date: 2024-11-06)

Primary citation

Scarff, C.A.,Thompson, R.F.,Newlands, G.F.J.,Jamson, A.H.,Kennaway, C.,da Silva, V.J.,Rabelo, E.M.,Song, C.F.,Trinick, J.,Smith, W.D.,Muench, S.P.
Structure of the protective nematode protease complex H-gal-GP and its conservation across roundworm parasites.
Plos Pathog., 16:e1008465-e1008465, 2020

PubMed Abstract: Roundworm parasite infections are a major cause of human and livestock disease worldwide and a threat to global food security. Disease control currently relies on anthelmintic drugs to which roundworms are becoming increasingly resistant. An alternative approach is control by vaccination and 'hidden antigens', components of the worm gut not encountered by the infected host, have been exploited to produce Barbervax, the first commercial vaccine for a gut dwelling nematode of any host. Here we present the structure of H-gal-GP, a hidden antigen from Haemonchus contortus, the Barber's Pole worm, and a major component of Barbervax. We demonstrate its novel architecture, subunit composition and topology, flexibility and heterogeneity using cryo-electron microscopy, mass spectrometry, and modelling. Importantly, we demonstrate that complexes with the same architecture are present in other Strongylid roundworm parasites including human hookworm. This suggests a common ancestry and the potential for development of a unified hidden antigen vaccine.

PubMed: 32271834
DOI: 10.1371/journal.ppat.1008465

Experimental method

ELECTRON MICROSCOPY (4.5 Å)