6RMG

Structure of PTCH1 bound to a modified Hedgehog ligand ShhN-C24II

6RMG の概要

• エントリーDOI: 10.2210/pdb6rmg/pdb
• EMDBエントリー: 4936
• 分子名称: Protein patched homolog 1,GFP-like fluorescent chromoprotein FP506, related, Sonic hedgehog protein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: patched, ptch1, hedgehog, shhn, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 203194.08

構造登録者

Korkhov, V.M.,Qi, C. (登録日: 2019-05-06, 公開日: 2019-10-09, 最終更新日: 2024-11-20)

主引用文献

Qi, C.,Di Minin, G.,Vercellino, I.,Wutz, A.,Korkhov, V.M.
Structural basis of sterol recognition by human hedgehog receptor PTCH1.
Sci Adv, 5:eaaw6490-eaaw6490, 2019

PubMed Abstract: Hedgehog signaling is central in embryonic development and tissue regeneration. Disruption of the pathway is linked to genetic diseases and cancer. Binding of the secreted ligand, Sonic hedgehog (ShhN) to its receptor Patched (PTCH1) activates the signaling pathway. Here, we describe a 3.4-Å cryo-EM structure of the human PTCH1 bound to ShhN, a modified hedgehog ligand mimicking its palmitoylated form. The membrane-embedded part of PTCH1 is surrounded by 10 sterol molecules at the inner and outer lipid bilayer portion of the protein. The annular sterols interact at multiple sites with both the sterol-sensing domain (SSD) and the SSD-like domain (SSDL), which are located on opposite sides of PTCH1. The structure reveals a possible route for sterol translocation across the lipid bilayer by PTCH1 and homologous transporters.

PubMed: 31555730
DOI: 10.1126/sciadv.aaw6490

実験手法

ELECTRON MICROSCOPY (3.4 Å)